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BioTracker

End-to-end support for eDNA analysis: Library preparation, Amplicon Sequencing and Bioinformatics.

“From sample to publication, with you”

With BioTracker, we aim to make metabarcoding analysis accessible and reliable, transforming complex data into clear and usable information. We support researchers at every stage, from sample preparation to the publication of results. The service is carried out by a team with solid, hands-on experience in multidisciplinary fields such as microbiology, ecology, and statistics.

We are able to research, adapt, and implement a variety of customized and emerging techniques to meet our clients' needs.

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After a free consultation and design phase, we get ready to receive your samples, such as soil, water, and plant tissues.

1. Sample delivery

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After receiving the samples, we extract the environmental DNA and prepare it for sequencing.

2. Library preparation

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Sequencing of the libraries is performed on the Ilumina MiSeq platform.

3. Illumina sequencing

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4. Bioinformatic analysis and Reporting

We process sequencing data and provide a clear report on the composition of environmental samples.

Together, we'll interpret the results to help you draw meaningful conclusions aligned with your project goals.

Library Preparation and Illumina Sequencing

Our services are modular: clients may submit raw samples, already-extracted DNA, or amplicons ready for sequencing. In addition to commonly used primers such as 16S and ITS, we can also work with primers for other markers upon explicit request from the client.

The price refers to the complete package, but can be adjusted for individual phases.

The unit price may be reduced if a larger number of samples is provided.

1. DNA extraction

2. DNA amplification

3. Amplicon purification

4. Sequencing

60

+ VAT
per sample

Bioinformatic Analysis Service

The pipelines used by our team are continuously updated to ensure increasingly accurate results. Taxonomic assignment is performed by comparing multiple databases to provide more reliable information. For certain specific taxa, we also use customized databases to deliver a more comprehensive classification.

The unit price may be reduced if a larger number of samples is provided.

Ready-to-Use Raw Data

Processing of FastQ files received from the sequencing platform (including demultiplexing, denoising, clustering, quality filtering, and taxonomic assignment).
Deliverables: Final report, file with taxonomic classification, ASV abundance matrix and Phylogenetic tree.

(Turnaround time 2-3 days)

13

+ VAT
per sample

Statistical Analysis and Visualization

Implementation of standard analytical approaches commonly adopted in scientific studies. 

Deliverables:  Alpha and Beta diversity analyses with corresponding visualizations, Taxa distribution plots, statistical analysis and plot of differential abundance, statistical analysis and plot of functional differences.

(Turnaround time 1 week)

25 

+ VAT
per sample

Tailored Scientific Support

Inclusion of specific analyses upon client request, assistance with publishing data in scientific journals (writing materials and methods, preparing figures in accordance with journal guidelines).

(Turnaround time may vary depending on client requests)

Custom price

Contacts

We offer various channels to provide support:

  • Email for formal updates and document exchange.

  • Video calls for in-depth discussions and scientific consultation.

  • WhatsApp chat for quick questions and immediate communication.

With BioTracker, we offer you a comprehensive, flexible, and personalized service. Contact us for a free consultation and discover how we can support your research projects.

mail:

 biotracker.lab@nature4.org

whatsapp and phone: 

+39 329 9334267

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